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July 21, 2005Synapse

Possible role of mitochondria in posttetanic potentiation of GABAergic synaptic transmission in rat neocortical cell cultures

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Authors

MSMaksim StorozhukBogomoletz Institute of Physiology SIS. Y. Ivanova ПБП. М. БалабанInstitute of Higher Nervous Activity and Neurophysiology

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In vitro electrophysiological study demonstrates mitochondrial calcium turnover mediates posttetanic potentiation in rat neocortical GABAergic synapses, suggesting presynaptic metabolic regulation.

Key Points

To determine whether mitochondria contribute to posttetanic potentiation of GABAergic synaptic transmission at central synapses in rat neocortical cell cultures.
Recorded inhibitory postsynaptic currents (IPSCs) using whole-cell patch-clamp techniques in cultured rat neocortical neurons under local extracellular stimulation.
Evoked posttetanic potentiation (PTP) using high-frequency tetanic stimulation (30 Hz, 4 s) and evaluated presynaptic mechanisms via coefficient of variation and paired-pulse ratios.
Inhibited mitochondrial Ca2+ uptake and efflux using carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 1–2 µM) and tetraphenylphosphonium ions (TPP+, 10 µM).
Tetanic stimulation elicited an increase in IPSC amplitude lasting approximately 1 minute, accompanied by decreases in both the coefficient of variation and the paired-pulse ratio.
Treatment with CCCP (1–2 µM) or TPP+ (10 µM) substantially decreased or eliminated PTP, identifying mitochondrial Ca2+ turnover as an essential presynaptic mechanism.

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Storozhuk et al. (2005) studied this question.

synapsesocial.com/papers/6a702f72660549caf2c4bf92 https://doi.org/10.1002/syn.20186

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Implication

In vitro electrophysiological study demonstrates mitochondrial calcium turnover mediates posttetanic potentiation in rat neocortical GABAergic synapses, suggesting presynaptic metabolic regulation.

Possible role of mitochondria in posttetanic potentiation of GABAergic synaptic transmission in rat neocortical cell cultures

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Implication

Key Points

Cite This Study

Possible role of mitochondria in posttetanic potentiation of GABAergic synaptic transmission in rat neocortical cell cultures

Discussion

Member takes

Implication

Key Points

Cite This Study

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