What question did this study set out to answer?

Investigate whether anti-DNA autoantibodies directly bind to glomerular antigens and trigger renal dysfunction independently of preformed circulating immune complexes.

May 1, 1989The Journal of ImmunologyOpen Access

Anti-DNA antibodies bind directly to renal antigens and induce kidney dysfunction in the isolated perfused rat kidney.

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Investigate whether anti-DNA autoantibodies directly bind to glomerular antigens and trigger renal dysfunction independently of preformed circulating immune complexes.
Administered mouse monoclonal anti-DNA IgG or human polyclonal anti-DNA IgG from patients with lupus nephritis into isolated perfused rat kidneys.
Measured urinary albumin excretion rates and inulin clearance over 160 minutes with and without complement addition or DNA preincubation.
Mouse anti-DNA IgG increased albumin excretion to 2301 ± 734 µg/min versus 85 ± 21 µg/min in controls (p < 0.001) and reduced inulin clearance to 0.17 ± 0.02 ml/min versus 0.28 ± 0.09 ml/min (p < 0.05).
Human anti-DNA IgG supplemented with plasma complement significantly increased albumin excretion to 1115 ± 195 µg/min (p < 0.02), whereas preincubation with DNA completely prevented antibody binding and physiological damage.

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Implication

Experimental study demonstrates direct anti-DNA antibody binding induces proteinuria in isolated rat kidneys, suggesting a direct role in lupus nephritis.

Cite This Study

Raz et al. (1989) studied this question.

synapsesocial.com/papers/6a7b627177336a1bae032b77 https://doi.org/10.4049/jimmunol.142.9.3076

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Implication

Experimental study demonstrates direct anti-DNA antibody binding induces proteinuria in isolated rat kidneys, suggesting a direct role in lupus nephritis.

Anti-DNA antibodies bind directly to renal antigens and induce kidney dysfunction in the isolated perfused rat kidney.

Key Points

Discussion

Implication

Cite This Study

Anti-DNA antibodies bind directly to renal antigens and induce kidney dysfunction in the isolated perfused rat kidney.

Key Points

Discussion

Implication

Cite This Study

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