June 26, 2020Circulation Heart Failure
Virome Sequencing in Patients With Myocarditis
Key result
Virome Capture Sequencing detected no relevant amounts of previously implicated viruses in cardiac tissue from giant cell myocarditis or blood from other types of myocarditis.
Why the study?
Viruses have not been implicated in giant cell myocarditis, and efforts to detect myocarditis-related viruses in more accessible samples such as peripheral blood have been unsuccessful.
Does Virome Capture Sequencing for Vertebrate Viruses detect implicated viruses in blood or cardiac tissue samples of patients with myocarditis?
Population
33 patients with myocarditis
Comparison
VirCapSeq-VERT screening across blood and cardiac tissue samples
Design
Observational cross-sectional virome sequencing study
Authors
Discussion
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Member takes
Implication
No pathogenic viruses detected in GCM by advanced sequencing; leaves open nonviral triggers and need for larger confirmatory studies.
Study Design
Type
Observational (n=33)
Structured PICO
Does Virome Capture Sequencing for Vertebrate Viruses detect implicated viruses in blood or cardiac tissue samples of patients with myocarditis?
P
Population
33 patients with myocarditis (including 9 with giant cell myocarditis and 4 with fulminant myocarditis) evaluated for viral sequences in blood and cardiac tissue.
E
Exposure
Virome Capture Sequencing for Vertebrate Viruses (VirCapSeq-VERT)
O
Outcome
Detection of viral nucleic acids in blood and cardiac tissue samplessurrogate
Comprehensive virome sequencing did not detect relevant amounts of previously implicated viruses in cardiac tissue from giant cell myocarditis or blood from other myocarditis types, suggesting that if viruses are involved in GCM, the mechanism is likely indirect.
Cite This Study
Heidecker et al. (2020) conducted an observational in Myocarditis (n=33). Virome Capture Sequencing for Vertebrate Viruses (VirCapSeq-VERT) was evaluated on Detection of viruses in blood and cardiac tissue. Virome Capture Sequencing detected no relevant amounts of previously implicated viruses in cardiac tissue from giant cell myocarditis or blood from other types of myocarditis.