Key result
Bariatric surgery in obese patients improved oxidative damage and reduced DNA double-strand breaks over a one-year follow-up compared to pre-operative levels.
Why the study?
Obesity promotes chronic inflammation and oxidative stress, but the extent of cell membrane oxidation and DNA damage in obese patients before and after bariatric surgery required evaluation.
Does bariatric surgery reduce cell membrane oxidation and DNA damage in obese patients?
Cohort
Does bariatric surgery reduce cell membrane oxidation and DNA damage in obese patients?
Bariatric surgery is associated with a reduction in systemic oxidative stress and DNA damage in obese patients at one year post-operation.
Answers are grounded in this paper and cite their sources.
Bariatric surgery was associated with reduced oxidative DNA damage; hypothesis-generating and should not yet change practice pending randomized trials.
The pathogenesis of obesity and related comorbidities has long been associated with oxidative stress. The excess of adipose tissue contributes to the production of free radicals that sustain both a local and a systemic chronic inflammatory state, whereas its reduction can bring to an improvement in inflammation and oxidative stress. In our work, using the fluorescent lipid probe BODIPY® 581/591 C11 and the γH2AX foci assay, a well-known marker of DNA double strand breaks (DSB), we evaluated the extent of cell membrane oxidation and DNA damage in peripheral blood lymphocytes of normal weight (NW) controls and obese patients sampled before and after bariatric surgery. Compared to NW controls, we observed a marked increase in both the frequencies of oxidized cells or nuclei exhibiting phosphorylation of histone H2AX in preoperatory obese patients. After bariatric surgery, obese patients, resampled over one-year follow-up, improved oxidative damage and reduced the presence of DSB. In conclusion, the present study highlights the importance for obese patients undergoing bariatric surgery to also monitor these molecular markers during their postoperative follow-up.
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Chiaramonte et al. (2023) conducted a cohort in Obesity. Bariatric surgery vs. Normal weight controls and pre-surgery baseline was evaluated on Cell membrane oxidation and DNA double strand breaks (DSB) in peripheral blood lymphocytes. Bariatric surgery in obese patients improved oxidative damage and reduced DNA double-strand breaks over a one-year follow-up compared to pre-operative levels.
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