What question did this study set out to answer?

To examine the physiological twitch characteristics and biochemical properties of isolated myosin and sarcoplasmic reticulum across feline fast, slow, and mixed hind limb muscles.

October 1, 1978Journal of Cellular Physiology

Morphological and biochemical correlates of skeletal muscle contractility in the cat. II. Physiological and biochemical studies

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Authors

WWW B Van WinkleUniversity of Hagen MEMark L. EntmanHeart Failure & Transplant EBEdward P. BornetBaylor College of Medicine

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Implication

Myosin ATPase and mobility differ by fiber type in cat muscle; leaves open isoform roles in human cardiac contractility.

Key Points

To examine the physiological twitch characteristics and biochemical properties of isolated myosin and sarcoplasmic reticulum across feline fast, slow, and mixed hind limb muscles.
Analyzed isometric twitch characteristics in cat caudofemoralis (fast-twitch), soleus (slow-twitch), and tibialis anterior (mixed gradient) muscles.
Isolated and biochemically characterized myosin ATPase activity and electrophoretic mobility, alongside sarcoplasmic reticulum calcium binding and uptake rates.
Separated the mixed-fiber tibialis anterior muscle into fast- and slow-twitch components via selective nerve stimulation.
Caudofemoralis myosin demonstrated 2- to 3-fold higher ATPase activity and greater intact electrophoretic mobility than soleus myosin, though both contained three light chains.
Caudofemoralis sarcoplasmic reticulum exhibited faster calcium binding and uptake kinetics with a higher density of fast-binding sites than soleus, matching their twitch relaxation differences.
Isolated myosin and sarcoplasmic reticulum fractions from selectively stimulated tibialis anterior components displayed distinct biochemical properties consistent with dual-function fiber gradients.

Cite This Study

Winkle et al. (1978) studied this question.

synapsesocial.com/papers/6a7bed2e351d91d38cc63e13 https://doi.org/10.1002/jcp.1040970111

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Morphological and biochemical correlates of skeletal muscle contractility in the cat. II. Physiological and biochemical studies

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Key Points

Cite This Study

Morphological and biochemical correlates of skeletal muscle contractility in the cat. II. Physiological and biochemical studies

Discussion

Member takes

Implication

Key Points

Cite This Study

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