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July 1, 1998AJP Cell Physiology

Regulation of Na+/Ca2+exchange activity by cytosolic Ca2+ in transfected Chinese hamster ovary cells

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Population

Transfected Chinese hamster ovary cells stably expressing the bovine cardiac Na+/Ca2+ exchanger and cells…

Comparison

Varying cytosolic Ca2+ concentrations using… vs Cells expressing a Ca2+-insensitive mutant of…

Design

Preclinical

Authors

YFYu FangHefei University of TechnologyMCMadalina CondrescuRutgers, The State University of New JerseyJRJohn P. ReevesRutgers, The State University of New Jersey

Discussion

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Implication

Challenges patch-based NCX Ca2+ sensitivity estimates; leaves open relevance to intact human cardiomyocyte Ca2+ handling.

Structured PICO

P
Population
Transfected Chinese hamster ovary cells stably expressing the bovine cardiac Na+/Ca2+ exchanger (CK1.4 cells) and cells expressing a Ca2+-insensitive mutant of the exchanger
I
Intervention
Varying cytosolic Ca2+ concentrations ([Ca2+]i) using ionomycin and EGTA or added CaCl2
C
Comparator
Cells expressing a Ca2+-insensitive mutant of the exchanger
O
Outcome
Rate of Ba2+ influx as a measure of Na+/Ca2+ exchange activitysurrogate

The concentration for activation of Na+/Ca2+ exchange activity in whole cells is approximately an order of magnitude lower than that reported for excised membrane patches.

Cite This Study

Fang et al. (1998) studied this question.

synapsesocial.com/papers/6a7beef2a540d02696c2ae30https://doi.org/10.1152/ajpcell.1998.275.1.c50
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Regulation of the cardiac Na(+)-Ca2+ exchanger by Ca2+. Mutational analysis of the Ca(2+)-binding domain.1995 · 248 citations
  2. 2Sodium-calcium exchange current. Dependence on internal Ca and Na and competitive binding of external Na and Ca.1989 · 216 citations
  3. 3Steady-state and dynamic properties of cardiac sodium-calcium exchange. Sodium-dependent inactivation.1992 · 304 citations
  4. 4Phosphorylation-dependent Regulation of Cardiac Na+/Ca2+ Exchanger via Protein Kinase C1996 · 189 citations
  5. 5Na‐Ca Exchange and Ca Fluxes during Contraction and Relaxation in Mammalian Ventricular Muscle<sup>a</sup>1996 · 121 citations