January 1, 1985Journal of Clinical InvestigationOpen Access

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.

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Population

Omental adipose tissue from 45 healthy subjects under the age of 50 undergoing elective surgery for…

Comparison

In vitro exposure to 10% fetal bovine serum… vs Control cultures without serum, insulin, or IGF1.

Design

Preclinical

Follow-up

up to 72 hours

Authors

PKPhilip A. KernUniversity of Kentucky SMSarah MarshallBrighton and Sussex Medical School RERobert H. EckelGeneral / Preventive / Lipids

Discussion

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Implication

Should not change clinical lipid management; hypothesis-generating for IGF1-mediated LPL regulation in adipocytes.

Structured PICO

Population

Omental adipose tissue from 45 healthy subjects (39 females, 6 males) under the age of 50 undergoing elective surgery for nonmalignant conditions.

Intervention

In vitro exposure to 10% fetal bovine serum, insulin (up to 400 ng/ml), insulin-like growth factor-1 (IGF1), and heparin.

Comparator

Control cultures without serum, insulin, or IGF1.

Outcome

Lipoprotein lipase (LPL) activity (secreted into culture medium, released by heparin, and extracted from cells).surrogate

Isolated human adipocytes produce and regulate lipoprotein lipase, with activity stimulated by serum, adiposity, and pharmacologic concentrations of insulin likely mediated through the IGF1 receptor.

Cite This Study

Kern et al. (1985) studied this question.

synapsesocial.com/papers/6a7c3a2dd51985d5a1c2a07f https://doi.org/10.1172/jci111675

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Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider