What question did this study set out to answer?

To investigate the catalytic enzyme fractions, required cofactors, and intermediate reactions involved in the biosynthesis of long-chain fatty acids from acetyl-CoA.

June 1, 1961Journal of Biological ChemistryOpen Access

Studies on the Mechanism of Fatty Acid Synthesis

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Authors

RBRubin BresslerUniversity of Arizona SWSalih J. WakilBaylor College of Medicine

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Implication

Biochemical analysis uncovers the catalytic role of bicarbonate and malonyl-CoA in hepatic fatty acid synthesis, elucidating the multi-step pathway of palmitate assembly.

Key Points

To investigate the catalytic enzyme fractions, required cofactors, and intermediate reactions involved in the biosynthesis of long-chain fatty acids from acetyl-CoA.
Fractionated avian and mammalian liver protein extracts to isolate the catalytic enzyme components required for fatty acid synthesis.
Assayed the cofactor dependencies of palmitic acid synthesis using radiolabeled bicarbonate, acetyl-CoA, ATP, Mn++, and reduced triphosphopyridine nucleotide.
Demonstrated that palmitic acid synthesis requires two distinct liver protein fractions along with ATP, Mn++, bicarbonate, and reduced triphosphopyridine nucleotide.
Established that bicarbonate acts catalytically in fatty acid synthesis without incorporating carbon atoms into the final palmitic acid product.
Identified malonyl-CoA as the initial reaction intermediate generated through the biotin-dependent carboxylation of acetyl-CoA by acetyl-CoA carboxylase.

Cite This Study

Bressler et al. (1961) studied this question.

synapsesocial.com/papers/6a7c5120d501feb4353bea94 https://doi.org/10.1016/s0021-9258(19)63278-3

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Implication

Biochemical analysis uncovers the catalytic role of bicarbonate and malonyl-CoA in hepatic fatty acid synthesis, elucidating the multi-step pathway of palmitate assembly.

Studies on the Mechanism of Fatty Acid Synthesis

Discussion

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Implication

Key Points

Cite This Study

Studies on the Mechanism of Fatty Acid Synthesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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