What question did this study set out to answer?

To identify and clone the enzyme responsible for reducing the Δ11(13) double bond during artemisinin biosynthesis in Artemisia annua glandular trichomes.

May 22, 2008Journal of Biological ChemistryOpen Access

The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin in Artemisia annua

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To identify and clone the enzyme responsible for reducing the Δ11(13) double bond during artemisinin biosynthesis in Artemisia annua glandular trichomes.
Investigated flower bud extracts of A. annua using partial protein purification, mass spectrometry, and expressed sequence tag analysis to isolate candidate cDNA clones.
Characterized recombinant enoate reductase enzyme substrate specificity and co-expressed the candidate gene with four other artemisinin pathway genes in yeast.
Isolated and cloned Dbr2, an enoate reductase family gene showing high sequence similarity to plant 12-oxophytodienoate reductases and preferential expression in glandular trichomes.
Demonstrated that recombinant Dbr2 specifically reduces artemisinic aldehyde, with minor activity on small α,β-unsaturated carbonyls.
Engineered yeast expressing Dbr2 alongside four pathway enzymes, resulting in the successful metabolic accumulation of dihydroartemisinic acid.

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Implication

Molecular study uncovers artemisinic aldehyde reductase Dbr2 in Artemisia annua trichomes, highlighting its critical role in the antimalarial artemisinin pathway.

Cite This Study

Zhang et al. (2008) studied this question.

synapsesocial.com/papers/6a7c68520449e558d7c2efb4 https://doi.org/10.1074/jbc.m803090200

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Implication

Molecular study uncovers artemisinic aldehyde reductase Dbr2 in Artemisia annua trichomes, highlighting its critical role in the antimalarial artemisinin pathway.

The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin in Artemisia annua

Key Points

Discussion

Implication

Cite This Study

The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin in Artemisia annua

Key Points

Discussion

Implication

Cite This Study

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