Sex-dependent right ventricular hypertrophic gene changes after methamphetamine treatment in mice

Figures

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  Fig. 1. Methamphetamine (MA) or vehicle treatment in female mice had no effect on right ventricular systolic pressure (A) Heart rate (B) or right heart contractility (C and D). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 5–6).

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  Fig. 2. Methamphetamine or vehicle treatment in male mice had no effect on right ventricular systolic pressure (A) Heart rate (B) or right heart contractility (C and D). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 8 each).

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  Fig. 3. Methamphetamine or vehicle treatment in female mice did not affect left ventricular systolic pressure (A) Heart rate (B) or left heart contractility (C and D). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 4–6).

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  Fig. 4. Methamphetamine or vehicle treatment in male mice did not affect left ventricular systolic pressure (A) Heart rate (B) or left heart contractility (C and D). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 4–5).

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  Fig. 5. Expression of genes associated with PAH and fibrosis in lung tissue isolated from A) vehicle- (white) and methamphetamine- (black) treated female mice and B) vehicle- (white) and methamphetamine- (black) treated male mice. BMPR2 (Bone Morphogenetic Protein Receptor Type II), HTR1B (5-Hydroxytryptamine (Serotonin) Receptor 1B), TGFβR1 (Transforming Growth Factor Beta Receptor I), ESR 1 (Estrogen Receptor Alpha), ESR 2 (Estrogen Receptor Beta), GPER (G Protein-Coupled Estrogen Receptor 1), TGFβ1 (Transforming Growth Factor Beta 1), CYP1A1 (Cytochrome P450 Family 1 Subfamily A polypeptide 1),CYP1B1 (Cytochrome P450 Family 1 Subfamily B, Polypeptide 1), Col1a1 (collagen type I), Col3a1 (collagen type III), FN1 (fibronectin 1). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 6). *P < 0.05 vs. vehicle group.

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  Fig. 6. Expression of genes associated with PAH and fibrosis in the RV tissue isolated from A) vehicle- (white) and methamphetamine- (black) treated female mice and B) vehicle- (white) and methamphetamine- (black) treated male mice. ANP (natriuretic peptide type A), BNP (natriuretic peptide type B), BMPR2 (Bone Morphogenetic Protein Receptor Type II), HTR1B (5-Hydroxytryptamine (Serotonin) Receptor 1B), TGFβR1 (Transforming Growth Factor Beta Receptor I), TGFβ1 (Transforming Growth Factor Beta 1), Col1a1 (collagen type I), Col3a1 (collagen type III), FN1 (fibronectin 1), Smad 2 (SMAD family member 2), Smad 3 (SMAD family member 3), Smad 7 (SMAD family member 7), Smurf 1 (SMAD specific E3 ubiquitin protein ligase 1), Smurf 2 (SMAD specific E3 ubiquitin protein ligase 2). Results are expressed as mean ± standard error of the mean (S.E.M) (n = 6). *P < 0.05 vs. vehicle group.

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  Fig. 7. Pulmonary vascular immunostaining for A) the proliferation marker; proliferating cell nuclear antigen (PCNA) (n = 5–6), and for B) α-smooth muscle actin (α-SMA) (n = 6 each group). Results are expressed as mean ± standard error of the mean (S.E.M). Scale bars indicate 20 μm.