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May 1, 2020Physiological ReportsOpen Access

Pulmonary arterial banding in mice significantly decreased LV and RV ejection fraction and LV torsion, and increased LV perivascular fibrosis and altered gene expression.

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Why the study?

Pulmonary hypertension causes RV pressure overload, but research has focused on the RV while overlooking the LV, which mechanically assists RV contraction.

Does right ventricular pressure overload induced by pulmonary arterial banding cause biomechanical and gene expression changes in the left ventricle in mice?

Population

Nine male mice

Comparison

Pulmonary arterial banding (N = 4) vs sham surgery (N = 5)

Design

Animal experimental study

Follow-up

7 weeks

Key result

Pulmonary arterial banding in mice significantly decreased LV and RV ejection fraction and LV torsion, and increased LV perivascular fibrosis and altered gene expression.

Authors

VKVitaly O. KheyfetsMDMelanie J. DufvaMBMario Boehm

Discussion

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Member takes

Overview

LV changes in RV overload model merit human validation; leaves open biventricular therapeutic targets in PH.

Structured PICO

Does right ventricular pressure overload induced by pulmonary arterial banding cause biomechanical and gene expression changes in the left ventricle in mice?

P
Population
9 male mice subjected to pulmonary arterial banding or sham surgery and followed for 7 weeks.
I
Intervention
Pulmonary arterial banding (PAB) to induce right ventricular pressure overload
C
Comparator
Sham surgery
O
Outcome
Biomechanical and gene expression changes in the left ventricle (evaluated by cardiac MRI at 1, 4, and 7 weeks, and histology/RT-PCR at 7 weeks)surrogate

Right ventricular pressure overload induces significant biomechanical and transcriptional changes in the left ventricle, highlighting the LV as a potential therapeutic target in pulmonary hypertension.

Limitations

  • A direct causal relationship between changes in LV and RV contractile mechanics was not established.

Cite This Study

Kheyfets et al. (2020) studied Right ventricular pressure overload (n=9). Pulmonary arterial banding (PAB) vs. Sham surgery was evaluated on Biomechanical and gene expression changes in the left ventricle. Pulmonary arterial banding in mice significantly decreased LV and RV ejection fraction and LV torsion, and increased LV perivascular fibrosis and altered gene expression.

synapsesocial.com/papers/6a792acd06f2b779205adf44https://doi.org/10.14814/phy2.14347
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sex-Dependent Changes in Right Ventricular Gene Expression in Response to Pressure Overload in a Rat Model of Pulmonary Trunk Banding2020 · 10 citations
  2. 2Molecular and physiological characterization of RV remodeling in a murine model of pulmonary stenosis2008 · 123 citations
  3. 3Transcriptomic profiles of ischemic and non-ischemic right ventricular heart failure2025
  4. 4Differential Responses of the Right Ventricle to Abnormal Loading Conditions in Mice: Pressure vs. Volume Load2011 · 83 citations
  5. 5Hypertrophy of the right ventricle by pulmonary artery banding in rats: a study of structural, functional, and transcriptomics alterations in the right and left ventricles2023 · 2 citations