April 1, 1989The Journal of Immunology
Endotoxemia elicits increased circulating beta 2-IFN/IL-6 in man.
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Discussion
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Implication
Experimental trial demonstrates increased circulating interleukin-6 and acute-phase proteins following endotoxin challenge in humans, suggesting a key role in host defense.
Key Points
- To determine whether systemic bacterial endotoxin administration stimulates circulating levels and bioactivity of beta 2-interferon/interleukin-6 in humans.
- Administered a single intravenous bolus of bacterial lipopolysaccharide (endotoxin, 20 U/kg) to human subjects.
- Measured circulating immunoreactive beta 2-IFN/IL-6 levels and evaluated bioactivity using hepatocyte stimulation and B-cell differentiation assays over time.
- Tracked downstream acute-phase reactant response by measuring circulating C-reactive protein levels up to 20 hours post-challenge.
- Endotoxin administration induced a rapid monophasic rise in circulating immunoreactive beta 2-IFN/IL-6 and bioactivity, reaching peak levels of 4.1 to 27.5 ng/ml at 2 to 4 hours post-injection.
- Circulating C-reactive protein levels significantly increased 20 hours after the endotoxin challenge, following the initial peak in IL-6.
Cite This Study
Fong et al. (1989) studied this question.