December 16, 2002Biochemical JournalOpen Access

Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle

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Population

Intact and permeabilized (alpha-toxin skinned) smooth muscle strips

Comparison

Agonist stimulation vs Resting state and removal of agonist

Design

Preclinical

Authors

Discussion

Prioritizes CPI17 over MBS in smooth muscle models; leaves open translation to human cardiovascular disease.

Population

Intact and permeabilized (alpha-toxin skinned) smooth muscle strips

Intervention

Agonist stimulation (with or without Rho kinase inhibitor Y27632 and protein kinase C inhibitor GF109203x)

Comparator

Resting state and removal of agonist

Outcome

Changes in the phosphorylation of MBS at Thr-641 and CPI17 at Thr-38surrogate

In smooth muscle, CPI17 phosphorylation plays a more significant role in agonist-induced Ca2+-independent contraction than MBS phosphorylation.

Niiro et al. (2002) studied this question.

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