Dietary tryptophan augments cancer-associated venous thrombogenicity mitigated by indoleamine 2,3-dioxygenase 1 inhibition

Figures

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  Figure 1.. Development of a syngeneic colon cancer tumor model with deep vein thrombosis. (A) A group of 8- to 12-week-old C57BL/6 female mice were injected at the flank with 1 million colon cancer MC38 cells or vehicle. Age-matched mice were compared between the tumor-bearing and control mice. Upon tumors reaching a certain size (typically within 2-3 weeks; see “Methods”), IVC ligation was performed, and clots were harvested 48 hours thereafter. (B) Averages of tumor growth in individual mice injected with MC38 over 3 weeks. (C) Histological analysis of MC38 cells; H&E stain was used. (D) Immunohistochemistry (IHC) of tumors using β-catenin antibody are shown at 100× original magnification (scale bar, 100 μm). (E) A schematic figure of the partial IVC ligation (see “Methods”) and an intraoperative feature of the IVC ligation model. (F) Averages of clot weights normalized to body weight from both groups are shown. Error bars represent the standard error of the mean (SEM; n = 5 mice per group; P = .0016). (G) Paraffin-embedded sections of IVC were stained using anti-TF and anti-CD31 antibodies. Alexa Fluor secondary antibodies and DAPI were used. Images are representative of IVCs from 5 mice…

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  Figure 2.. Influence of high-protein diet in a syngeneic colon cancer tumor model. Age-matched C57BL/6 female mice were used as in Figure 1. (A) Averages of tumor volumes per group are shown. Error bars represent SEM. (B) Averages of normalized clot weights are shown. Two-factor analysis of variance (ANOVA; P = .0002). (C) Paraffin-embedded sections of IVC were stained using anti-TF and anti-CD31 antibodies. Alexa Fluor secondary antibodies and DAPI were used. Images are representative of IVCs from 5 mice in each group (scale bar, 100 μm). (D) ID was normalized to the surface area of the region of interest measured in microns by using ImageJ. Averages of ID normalized to surface area are shown. Error bars represent SEM. Two-factor ANOVA test (P = .0003). (E) IVCs were stained by using anti–PAI-1 and anti-CD31 antibodies. Alexa Fluor secondary antibodies and DAPI were used. Representative images from 5 mice in each group (scale bar, 100 μm). (F) PAI-1 expression was quantified as previously described. Averages of ID normalized to the surface area are shown. Error bars represent SEM. Two-factor ANOVA test (P = .0002; ∗∗P = .0255). #P < 0.05, ∗P < 0.05.

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  Figure 3.. Dietary Trp alters the risk of venous thrombogenicity in a syngeneic colon cancer model. Age-matched C57BL/6 female mice were used as in Figure 1. (A) Averages of tumor growth in all 3 dietary groups are shown. Error bars represent standard deviation. (B) Representative harvested tumors from the 3 groups are shown. (C) Averages of tumor weights in the 3 experimental groups are shown (zero Trp vs 0.2% Trp [normal] vs 1.2% Trp [high]). Error bars represent SEM. (D) Representative images of H&E-stained tumor tissue sections are shown at 40× and 100× original magnifications (scale bars, 25 μm [40×] and 50 μm [100×]). (E) Averages of normalized clot weights in female mice under 6 experimental conditions. Two-factor ANOVA showed P value <.001. Error bars represent SEM. (F) Averages of normalized clot weights in male mice under 6 experimental conditions. The number of mice analyzed under each condition is denoted by dots in each bar graph. Error bars represent SEM. Two-factor ANOVA test showed P value <.001. ns, nonsignificant. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.0001, ###P < 0.0001, ####P < 0.0001.

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  Figure 4.. The expressions of procoagulant and anticoagulant proteins are altered in the IVC of mice with colon cancer tumors and on a high Trp diet. Age-matched C57BL/6 female mice were used as in Figure 1. (A) Representative images of paraffin-embedded sections of IVC from mice with MC38 tumor exposed to a normal, zero, or high Trp diets (n = 10 female mice per group). IVC tissues were stained with indicated antibodies. DAPI was used for nuclear stain (scale bar, 100 μm). (B) Averages of normalized ID of TF normalized to surface area in each of the examined sections. Three images per IVC were analyzed in each mouse. Two-factor ANOVA test (P < .0001). (C) Representative images of tissue section of IVC stained with anti–PAI-1 and anti-CD31 antibodies (scale bar, 100 μm). (D) Averages of normalized ID of PAI-1 normalized to surface area in each of the examined section. ANOVA (P < .0001). (E) Representative images of paraffin-embedded sections of IVC from female mice with MC38 tumor exposed to a normal, zero, or high Trp diets, stained with TM and CD31 (n = 10 mice per group). DAPI was used for nuclear stain (scale bar, 100 μm). (F) Averages of normalized ID of TM expression in endothelial…

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  Figure 5.. Alterations in Trp metabolome in mice with syngeneic colon cancer tumors. (A) Average diet intake in grams was measured in 4 age-matched female mice subjected to different Trp diets over 4 days. The y-axis denotes the diet intake per day per mouse. Error bars represent SEM. (B-E) Average levels of Trp metabolites in 4 to 5 mice per group are shown. Error bars represent SEM. ANOVA was performed to compare all the groups. P = .0006 (Trp levels ANOVA); P = .0017 (Kyn ANOVA); P = .0147 (kynurenic acid ANOVA); indoxyl sulfate ANOVA was not significant. Student t test was performed to compare individual groups. For kynurenic acid, in the nontumor group, P = .0631 (between normal and high Trp groups); P = .0778 (between zero and high Trp groups). No significant differences were noted between different diets in the tumor groups. (F) A linear correlation of normalized clot weights with Kyn levels.

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  Figure 6.. IDO1 inhibition attenuates thrombosis in a mouse cancer model under different Trp diets. (A) IDO activity measured in the sera of mice with and without MC38 tumor. A group of age-matched 8- to 12-week-old C57BL/6 female mice were used for this experiment. The y-axis is depicted in a log scale. Error bars represent SEM. Student t test was used to compare the groups (P = .0037). (B) Clot weights measured under different diets, as described in Figure 3E, were plotted against values of IDO1 activity measured in the sera of mice described in panel A. A linear correlation between normalized clot weights and IDO activity levels. (C) Representative IF images of IVC from IVC of mice on different Trp diets, stained with antibodies to IDO1 and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). (D) ID of IDO1 labeling normalized to a uniform surface area in all samples. ANOVA (P < .001). Student t test was used to compare groups. (E) Averages of tumor volume in all groups are shown. Error bars represent SEM. (F) Averages of clot weights normalized to the body weight across different groups of mice are shown. Error bars represent SEM. ANOVA was done to compare all groups; P < .0001.…

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  Figure 7.. IDO1 inhibition alters TF, PAI-1, and TM expression in IVC of mice with tumors exposed to a high Trp diet. (A) Representative images of tissue sections of IVC from MC38 tumor–bearing mice stained with TF and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). White arrowheads point to the TF expression on endothelial cells, and asterisk points to the subendothelial cells (B) Averages of normalized ID are shown. ANOVA (P < .0001). Student t test was used to compare groups. (C) Representative images of tissue sections of IVC from mice in different groups stained with PAI-1 and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). White arrowheads point to the PAI-1 expression on endothelial cells, and asterisk points to the subendothelial cells. (D) Averages of normalized ID are shown. ANOVA (P = .0036). Student t test was used to compare groups. (E) Representative images of tissue sections of IVC from MC38 tumor–bearing mice stained with TM and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). (F) Averages of normalized ID are shown. Data are representative of 5 age-matched female mice per group. ANOVA (P = .0015). Student t test was used to compare groups.…