What question did this study set out to answer?

To elucidate the biosynthetic mechanism and enzymatic processing steps responsible for producing cage-like tricyclic depsipeptides in bloom-forming cyanobacteria.

August 6, 2008Angewandte Chemie International EditionOpen Access

Ribosomal Synthesis of Tricyclic Depsipeptides in Bloom‐Forming Cyanobacteria

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Authors

NZNadine ZiemertGerman Center for Infection Research KIKeishi IshidaLeibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)ALAnton LiaimerUiT The Arctic University of Norway

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Implication

Genetic and chemical analysis reveals ribosomal synthesis and maturation pathways of microviridin depsipeptides in cyanobacteria, highlighting novel post-translational enzymology.

Key Points

To elucidate the biosynthetic mechanism and enzymatic processing steps responsible for producing cage-like tricyclic depsipeptides in bloom-forming cyanobacteria.
Conducted genetic and chemical analyses of microviridin biosynthetic gene clusters.
Utilized heterologous expression systems to evaluate the enzymatic conversion of precursor peptides.
Characterized post-translational modifications and peptide intermediates using MALDI-PSD mass spectrometry.
Identified that microviridin B and J protease inhibitors originate from ribosomally synthesized prepeptides rather than nonribosomal peptide synthetases.
Showed that ATP grasp ligases convert the linear precursor prepeptides into cross-linked tricyclic depsipeptides.
Demonstrated that a transporter peptidase facilitates the processing and maturation of the cage-like cyclized peptides.

Cite This Study

Ziemert et al. (2008) studied this question.

synapsesocial.com/papers/6a7d1abca3b6fd5035d5a2ac https://doi.org/10.1002/anie.200802730

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Implication

Genetic and chemical analysis reveals ribosomal synthesis and maturation pathways of microviridin depsipeptides in cyanobacteria, highlighting novel post-translational enzymology.

Ribosomal Synthesis of Tricyclic Depsipeptides in Bloom‐Forming Cyanobacteria

Discussion

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Implication

Key Points

Cite This Study

Ribosomal Synthesis of Tricyclic Depsipeptides in Bloom‐Forming Cyanobacteria

Discussion

Member takes

Implication

Key Points

Cite This Study

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