What question did this study set out to answer?

To elucidate the functional relationship between ARHGAP18 and YAP in mediating laminar flow-induced endothelial cell alignment and inflammatory signaling.

February 3, 2020Cell Communication and SignalingOpen Access

YAP and the RhoC regulator ARHGAP18, are required to mediate flow-dependent endothelial cell alignment

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Authors

PCPaul ColemanThe University of Sydney ALAngelina J. LayThe University of Sydney KTKa Ka TingThe University of Sydney

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Implication

Laboratory study reveals that ARHGAP18 and YAP mediate flow-dependent alignment in human endothelial cells and mice, indicating an athero-protective pathway against vascular inflammation.

Key Points

To elucidate the functional relationship between ARHGAP18 and YAP in mediating laminar flow-induced endothelial cell alignment and inflammatory signaling.
Depleted ARHGAP18 or YAP using siRNA in human endothelial cells cultured under athero-protective, laminar shear stress conditions.
Assessed YAP activity, nuclear localization, and junctional integrity via high-performance confocal microscopy in ARHGAP18 knockout mice.
Depletion of either ARHGAP18 or YAP disrupted endothelial cell alignment under high shear stress and stimulated NF-κB activation via p65 nuclear localization.
Loss of ARHGAP18 decreased overall YAP levels while promoting its nuclear translocation and target gene Cyr61 induction, whereas ARHGAP18 overexpression promoted YAP phosphorylation and inactivation.
Endothelial regions in ARHGAP18-deficient mice displayed loss of cell alignment, decreased VE-Cadherin at junctions, and nuclear localization of YAP linked to a pro-inflammatory phenotype.

Cite This Study

Coleman et al. (2020) studied this question.

synapsesocial.com/papers/6a7d329bd85f1805a31cd1f4 https://doi.org/10.1186/s12964-020-0511-7

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Implication

Laboratory study reveals that ARHGAP18 and YAP mediate flow-dependent alignment in human endothelial cells and mice, indicating an athero-protective pathway against vascular inflammation.

YAP and the RhoC regulator ARHGAP18, are required to mediate flow-dependent endothelial cell alignment

Discussion

Member takes

Implication

Key Points

Cite This Study

YAP and the RhoC regulator ARHGAP18, are required to mediate flow-dependent endothelial cell alignment

Discussion

Member takes

Implication

Key Points

Cite This Study

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