Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 1, 1999Journal of Biological ChemistryOpen Access

The Activity of Microsomal Triglyceride Transfer Protein Is Essential for Accumulation of Triglyceride within Microsomes in McA-RH7777 Cells

View Full Paper
Ask AI
Bookmark
Share

Population

Transfected McA-RH7777 cells expressing various apolipoprotein B (apoB) forms (B64, B72, B80, and B100)

Comparison

MTP inhibitor BMS-197636 and exogenous oleate vs Untreated cells or cells treated with brefeldin A

Design

Preclinical

Authors

YWYuwei WangZhejiang UniversityKTKhai TranUniversity of Da NangZYZemin YaoChina Academy of Space Technology

Discussion

Loading...

Member takes

Implication

MTP inhibition blocks VLDL assembly in animal microsomes; leaves open clinical translation to human dyslipidemia.

Structured PICO

P
Population
Transfected McA-RH7777 cells expressing various apolipoprotein B (apoB) forms (B64, B72, B80, and B100)
I
Intervention
MTP inhibitor BMS-197636 (0.05-0.5 microM) and exogenous oleate
C
Comparator
Untreated cells or cells treated with brefeldin A (0.2 microgram/ml)
O
Outcome
VLDL assembly and accumulation of triglyceride within microsomessurrogate

Microsomal triglyceride transfer protein (MTP) is essential for facilitating the accumulation of triglycerides within microsomes, a prerequisite for the post-translational assembly of TG-enriched VLDL.

Cite This Study

Wang et al. (1999) studied this question.

synapsesocial.com/papers/6a7d3df8d85f1805a31cd40dhttps://doi.org/10.1074/jbc.274.39.27793
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Apolipoprotein B Binding to Microsomal Triglyceride Transfer Protein Decreases with Increases in Length and Lipidation: Implications in Lipoprotein Biosynthesis1997 · 58 citations
  2. 2In Vitro Reconstitution of Assembly of Apolipoprotein B48-containing Lipoproteins1997 · 29 citations
  3. 3Carboxyl-terminal truncation impairs lipid recruitment by apolipoprotein B100 but does not affect secretion of the truncated apolipoprotein B-containing lipoproteins.1994 · 84 citations
  4. 4PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT1951 · 318,705 citations
  5. 5The Microsomal Triglyceride Transfer Protein Catalyzes the Post-translational Assembly of Apolipoprotein B-100 Very Low Density Lipoprotein in McA-RH7777 Cells1998 · 127 citations