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26S RNA predominates in Sindbis polysomes; leaves open mechanisms of viral mRNA selection for further study.
Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.
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Deborah Bernhardt Mowshowitz (1973) studied this question.
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