What question did this study set out to answer?

To investigate whether non-pungent capsinoids activate other transient receptor potential (TRP) channels beyond their established target, TRPV1.

February 10, 2012British Journal of PharmacologyOpen Access

Activation of transient receptor potential A1 by a non‐pungent capsaicin‐like compound, capsiate

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Authors

KSKenji ShintakuNational Institutes of Natural Sciences KUKunitoshi UchidaUniversity of Shizuoka YSYoshiro SuzukiIwate Medical University

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Implication

In vitro study demonstrates activation of TRPA1 channels by capsinoids in mouse sensory neurons, suggesting an alternative pathway for non-pungent pepper metabolic benefits.

Key Points

To investigate whether non-pungent capsinoids activate other transient receptor potential (TRP) channels beyond their established target, TRPV1.
Assessed three capsinoids—capsiate, dihydrocapsiate, and nordihydrocapsiate—and their breakdown products using calcium imaging and whole-cell patch-clamp electrophysiology.
Tested channel responses in TRP-expressing HEK293T cells and primary cultured mouse dorsal root ganglion neurons, including evaluations with specific TRPA1 antagonists and mutant channels.
Capsinoids activated TRPA1 in both cell types with a lower potency (higher EC50) than TRPV1, and capsinoid-induced currents were fully blocked by a specific TRPA1 antagonist.
Capsinoid degradation products failed to activate TRPA1, and channel activation proceeded without involving standard cysteine covalent modification or zinc-coordinating residues.

Cite This Study

Shintaku et al. (2012) studied this question.

synapsesocial.com/papers/6a7d4c7cff1a536db837d702 https://doi.org/10.1111/j.1476-5381.2011.01634.x

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Implication

In vitro study demonstrates activation of TRPA1 channels by capsinoids in mouse sensory neurons, suggesting an alternative pathway for non-pungent pepper metabolic benefits.

Activation of transient receptor potential A1 by a non‐pungent capsaicin‐like compound, capsiate

Discussion

Member takes

Implication

Key Points

Cite This Study

Activation of transient receptor potential A1 by a non‐pungent capsaicin‐like compound, capsiate

Discussion

Member takes

Implication

Key Points

Cite This Study

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