What question did this study set out to answer?

To identify, map, and evaluate the phylogenetic conservation of the specific epitope recognized by the cardiac troponin T monoclonal antibody mAb 13-11.

May 1, 1992Journal of Biological ChemistryOpen Access

A cardiac troponin T epitope conserved across phyla.

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To identify, map, and evaluate the phylogenetic conservation of the specific epitope recognized by the cardiac troponin T monoclonal antibody mAb 13-11.
Generated monoclonal antibody mAb 13-11 against rabbit cardiac troponin T and evaluated reactivity across cardiac and skeletal tissues in fish, birds, frogs, and mammals.
Mapped the target epitope using stepwise cyanogen bromide digestion alongside slot-blot and enzyme-linked immunosorbent assays (ELISA) of synthetic peptides.
Mapped the mAb 13-11 epitope to a conserved domain (PFMPNLVPPKI) encoded by the 5' region of cardiac gene exon 10, with ELISA demonstrating highest binding affinity to a 17-residue sequence.
Detected the epitope in cardiac troponin T across fish, birds, and mammals (absent in frogs), as well as transiently in immature skeletal muscle, but observed complete absence in adult skeletal muscle.

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Implication

Laboratory study demonstrates cross-species conservation of a cardiac troponin T epitope, suggesting an essential role in heart-specific myofilament structure and function.

Cite This Study

Malouf et al. (1992) studied this question.

synapsesocial.com/papers/6a7d4da500c5c9efbcaa17ff https://doi.org/10.1016/s0021-9258(19)50418-5

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Implication

Laboratory study demonstrates cross-species conservation of a cardiac troponin T epitope, suggesting an essential role in heart-specific myofilament structure and function.

A cardiac troponin T epitope conserved across phyla.

Key Points

Discussion

Implication

Cite This Study

A cardiac troponin T epitope conserved across phyla.

Key Points

Discussion

Implication

Cite This Study

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