What question did this study set out to answer?

To determine the kinetic mechanisms by which beta-adrenergic agonists and cyclic AMP stimulators regulate glucose transport in rat and human fat cells.

November 1, 1983Journal of Biological ChemistryOpen Access

The regulation of glucose transport by cAMP stimulators via three different mechanisms in rat and human adipocytes.

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Authors

AKAtsunori KashiwagiShiga Medical Center THT P HuecksteadtIndiana Orthopaedic Hospital JFJames E. FoleyHarvard University

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Implication

Laboratory study reveals three distinct mechanisms regulating glucose transport via cAMP stimulators in rat and human adipocytes, indicating complex metabolic control of sugar uptake.

Key Points

To determine the kinetic mechanisms by which beta-adrenergic agonists and cyclic AMP stimulators regulate glucose transport in rat and human fat cells.
Assessed kinetics of 3-O-methylglucose (MG) transport in isolated rat adipocytes, human adipocytes, and isolated rat plasma membrane vesicles.
Evaluated transport under basal and insulin-stimulated conditions using varying concentrations of L-isoproterenol, adenosine deaminase, dibutyryl cAMP (Bt2cAMP), isobutylmethylxanthine (IBMX), and forskolin.
In rat adipocytes, low concentrations of L-isoproterenol (10–25 nM) stimulated basal MG transport by increasing Vmax, whereas higher concentrations (0.5–2 μM) or addition of adenosine deaminase inhibited transport by decreasing Vmax.
In human adipocytes, L-isoproterenol stimulated basal MG transport without suppressing maximal rates, whereas adenosine deaminase plus L-isoproterenol or Bt2cAMP suppressed maximum insulin-stimulated transport by 40%.
Forskolin and IBMX directly inhibited glucose transport in plasma membrane vesicles and suppressed both basal and maximal MG transport by 85% in human adipocytes by decreasing Vmax and increasing apparent Km.

Cite This Study

Kashiwagi et al. (1983) studied this question.

synapsesocial.com/papers/6a7d5d2fda72e8de9c279bac https://doi.org/10.1016/s0021-9258(17)43971-8

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Implication

Laboratory study reveals three distinct mechanisms regulating glucose transport via cAMP stimulators in rat and human adipocytes, indicating complex metabolic control of sugar uptake.

The regulation of glucose transport by cAMP stimulators via three different mechanisms in rat and human adipocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

The regulation of glucose transport by cAMP stimulators via three different mechanisms in rat and human adipocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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