RNA‐Directed Packaging of Enzymes within Virus‐like Particles

Packaged molecular machines are available using dual expression vectors that guide the preparation of Qβ virus-like particles encapsulating multiple enzymes. Packaging is promoted by RNA aptamer sequences that bridge between the coat protein and a peptide tag fused to the desired cargo (see scheme). Peptidase E and luciferase were thus encapsulated and shown to be catalytically active inside the particle.