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May 1, 1992Endocrine Reviews

COUNTERPOINT: Molecular Analysis of the Angiotensin II Receptor*

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Population

Rat vascular angiotensin II receptor model and historical pharmacological studies on liver membranes

Design

Review

Authors

KBKenneth E. BernsteinCross-Cutting CardiologyRAR. Wayne AlexanderGeneral / Preventive / Lipids

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Implication

Supports ARB use in hypertension and HF; extends early receptor heterogeneity data to guide current subtype-specific RAS research.

Key Points

  • Detail the molecular isolation of complementary DNA encoding the rat vascular angiotensin II receptor and review the pharmacological evidence for receptor subtypes.
  • Cloned and isolated complementary DNA (cDNA) encoding the rat vascular angiotensin II receptor.
  • Assessed pharmacological heterogeneity using radioligand binding of [125I]angiotensin II, nonpeptidic antagonists, and dithiothreitol sensitivity.
  • Identified distinct receptor binding sites in liver membranes, including a high-affinity site (Kd 0.35 nM) sensitive to dithiothreitol and a low-affinity site (Kd 3.1 nM).
  • Demonstrated functional heterogeneity where angiotensin III exhibited greater potency for aldosterone production, while angiotensin II showed greater potency for blood pressure elevation.

Structured PICO

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Population
Rat vascular angiotensin II receptor model (cDNA isolation) and historical pharmacological studies on liver membranes

Provides historical and molecular context on the isolation of the cDNA encoding the rat vascular angiotensin II receptor, foundational for understanding the renin-angiotensin system.

Cite This Study

Bernstein et al. (1992) studied this question.

synapsesocial.com/papers/6a7d6e5614bfcdf15b6e54eehttps://doi.org/10.1210/edrv-13-2-381
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Induction of platelet-derived growth factor A-chain and c-myc gene expressions by angiotensin II in cultured rat vascular smooth muscle cells.1989 · 625 citations
  2. 2Angiotensin II induces smooth muscle cell proliferation in the normal and injured rat arterial wall.1991 · 743 citations
  3. 3Selective Amplification And Cloning Of Four New Members Of The G Protein-coupled Receptor Family1989 · 642 citations
  4. 4Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.1987 · 924 citations