The study demonstrates that recombinant polioviruses can express heterologous antigens while maintaining VP0 myristylation, though insert size and nature affect viral growth and stability.
Recombinant poliovirus vectors remain viable in animal models; leaves open optimization and clinical translation for antigen delivery.
Recombinant polioviruses expressing antigens from rotavirus, herpes simplex virus type 2, and hepatitis B virus were generated. Fusion of the heterologous polypeptides to the amino terminus of the poliovirus polyprotein did not prevent myristylation of VP0, suggesting a novel mechanism of myristylation for these recombinant viruses. The effects of the parental genetic background, different foreign sequences, and different insert sizes on growth characteristics were compared. Both the size and the nature of the heterologous sequence appeared to be factors influencing the growth and stability of recombinant polioviruses. All of the recombinants showed a temperature-sensitive phenotype, regardless of the genetic background (attenuated or wild type) from which they were derived. Preliminary studies with transgenic mice carrying the poliovirus receptor gene are discussed.
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Mattion et al. (1995) studied this question.
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