Purification of antigenically intact Ro ribonucleoproteins; biochemical and Immunological evidence that the 52-kD protein is not a Ro protein

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Population

HeLa cells and human anti-Ro sera

Comparison

Biochemical purification of Ro… vs Unfractionated HeLa cell extracts and…

Design

Preclinical

Authors

Discussion

Questions p52 inclusion in intact Ro RNPs; challenges prior models but leaves clinical relevance in autoimmunity open.

Population

HeLa cells and human anti-Ro sera

Intervention

Biochemical purification of Ro ribonucleoproteins (RNPs) using non-denaturing conditions

Comparator

Unfractionated HeLa cell extracts and unfractionated Ro RNPs

Outcome

Presence of 52-kD protein (p52) in purified Ro RNPs and immunoprecipitation capacity

The 52-kD protein is not a stable component of antigenically intact Ro RNPs, contrary to previous hypotheses.

Boire et al. (1995) studied this question.

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