Key Points
- Map five previously unassigned poliovirus proteins (5a, 7d, 8, 9b, and 10) onto the proteolytic processing pathway of the viral polyprotein.
- Analyzed precursor-product relationships and sister fragments generated during poliovirus polyprotein proteolytic cleavage.
- Protein 9b was identified as the sister fragment of VPg resulting from the cleavage of precursor P3-9 into P3-9b and VPg.
- Proteins 8 and 10 were mapped as sister fragments of X-related proteins (P2-3b yields P2-8 + P2-5b; P2-5b yields P2-10 + P2-X), while proteins 5a and 7d were localized to cleavages involving the 1b region.
- The assignments account for over 95% of total polioviral proteins, completing the mapping of major proteolytic processing pathways.
Structured PICO
PPopulationPoliovirus proteins
IInterventionMapping of proteolytic processing
OOutcomeLocation of previously unmapped proteins on the proteolytic processing map
The study completes the mapping of the major processing pathways of polioviral proteins.