New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites

View Full Paper

Ask AI

Bookmark

Population

Saccharomyces cerevisiae actin (9 site-specific mutations)

Comparison

Actin-binding drugs vs Wild-type yeast actin

Design

Preclinical

Authors

Discussion

Yeast actin mapping does not guide clinical practice; leaves open relevance to mammalian cardiac actin dynamics.

Population

Saccharomyces cerevisiae actin (9 site-specific mutations)

Intervention

Actin-binding drugs (latrunculin A, phalloidin, tolytoxin)

Comparator

Wild-type yeast actin

Outcome

Drug binding sites and nucleotide exchange ratessurrogate

Identifies specific binding sites for latrunculin A, phalloidin, and tolytoxin near the actin nucleotide binding pocket using yeast actin mutants.

Belmont et al. (1999) studied this question.

View Full Paper

Ask AI

Bookmark