June 12, 2013Scientific ReportsOpen Access

Agonist Activated PKCβII Translocation and Modulation of Cardiac Myocyte Contractile Function

Q: What is the clinical evidence from this study?

Study design: Other. Population: Heart failure (cellular model). Intervention: PKCβII gene transfer and Phenylephrine (PHE) vs. Control myocytes or PKCβDN-expressing myocytes. Primary outcome: Contractile function (shortening amplitude and rates).

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Key result

In adult rat cardiac myocytes, PKCβII up-regulation preserved contractile function in response to low-dose phenylephrine, accompanied by PKCβII translocation and complex downstream phosphorylation.

Population

Adult rat cardiac myocytes

Comparison

Phenylephrine in PKCβII-expressing myocytes… vs Phenylephrine in control and PKCβDN-expressing…

Design

Preclinical

Authors

HHHyosook HwangUlsan College DRDustin RobinsonMarshall University JRJulie B. Rogers

Discussion

Member takes

Implication

May support PKCβII-targeted strategies in early myocyte dysfunction; leaves open translation to human HF or therapy.

Structured PICO

Population

Isolated adult rat cardiac myocytes subjected to PKCβII gene transfer to study contractile function and downstream signaling.

Intervention

Phenylephrine (100 nM) in PKCβII-expressing myocytes, with or without LY379196 (30 nM)

Comparator

Phenylephrine (100 nM) in control and PKCβDN-expressing myocytes

Outcome

Contractile function (amplitude and rates of shortening and re-lengthening)surrogate

In adult rat cardiac myocytes, agonist-induced PKCβII activation preserves contractile function and triggers complex downstream signaling, providing insight into its role in heart failure.

Limitations

In vitro study using isolated rat myocytes, which may not fully replicate in vivo human heart failure.
Reliance on adenoviral gene transfer models.
Difficulty in capturing transient changes in phosphorylation levels during an agonist response.

Cite This Study

Hwang et al. (2013) studied Heart failure (cellular model). PKCβII gene transfer and Phenylephrine (PHE) vs. Control myocytes or PKCβDN-expressing myocytes was evaluated on Contractile function (shortening amplitude and rates). In adult rat cardiac myocytes, PKCβII up-regulation preserved contractile function in response to low-dose phenylephrine, accompanied by PKCβII translocation and complex downstream phosphorylation.

synapsesocial.com/papers/6a7da991fa78bd2571186bfd https://doi.org/10.1038/srep01971

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Agonist Activated PKCβII Translocation and Modulation of Cardiac Myocyte Contractile Function

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Agonist Activated PKCβII Translocation and Modulation of Cardiac Myocyte Contractile Function

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider