What question did this study set out to answer?

To isolate and characterize the biochemical and structural properties of high density lipoproteins in patients homozygous for Tangier disease.

July 1, 1977Journal of Clinical InvestigationOpen Access

Isolation and Characterization of an Abnormal High Density Lipoprotein in Tangier Disease

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To isolate and characterize the biochemical and structural properties of high density lipoproteins in patients homozygous for Tangier disease.
Isolated lipoproteins from the plasma of five patients homozygous for Tangier disease using preparative ultracentrifugation and agarose-column chromatography.
Characterized protein and particle composition via negative-stain electron microscopy, polyacrylamide gel electrophoresis, immunochemistry, and fluorescence spectroscopy.
Evaluated lipid-protein interactions through in vitro phospholipid reconstitution assays and 125I-labeled very low density lipoprotein exchange experiments.
Tangier high density lipoprotein fractions yielded abnormal spherical particles 55–75 Å in diameter containing apoprotein A-II as their sole protein constituent.
Apoprotein A-I and C apoproteins were completely absent from the isolated Tangier lipoprotein fraction.
Tangier apoprotein A-II retained normal amino acid composition and phospholipid-binding capability, but failure of C apoproteins to associate with high density fractions impairs triglyceride clearance.

Structured PICO

Population

5 patients homozygous for Tangier disease (familial high density lipoprotein deficiency) and normolipidemic control subjects.

Outcome

Morphological, chemical, and immunochemical characterization of high density lipoproteins (HDL).surrogate

Patients with Tangier disease lack normal HDL and instead possess an abnormal HDL particle containing only apoprotein A-II, which impairs C apoprotein metabolism and contributes to hypertriglyceridemia.

Limitations

Limited material available did not permit accurate determinations of minor lipid constituents such as phosphatidyl ethanolamine, phosphatidyl serine, lysophosphatidyl choline, and triglyceride.

Discussion

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Implication

Abnormal A-II HDL in Tangier disease may impair C apoprotein metabolism; leaves open its contribution to hypertriglyceridemia and need for targeted studies.

Cite This Study

Assmann et al. (1977) studied this question.

synapsesocial.com/papers/6a7dab95968fa26fcacb4d4b https://doi.org/10.1172/jci108761

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Implication

Abnormal A-II HDL in Tangier disease may impair C apoprotein metabolism; leaves open its contribution to hypertriglyceridemia and need for targeted studies.

Isolation and Characterization of an Abnormal High Density Lipoprotein in Tangier Disease

Key Points

Structured PICO

Limitations

Discussion

Implication

Cite This Study

Isolation and Characterization of an Abnormal High Density Lipoprotein in Tangier Disease

Key Points

Structured PICO

Limitations

Discussion

Implication

Cite This Study

Also Consider

Also Consider