What question did this study set out to answer?

To develop and evaluate a low-temperature, water-miscible plastic embedding method for high-resolution sectioning, histochemistry, and immunostaining of undecalcified bone.

January 1, 1980Stain Technology

Staining and Histochemistry of Undecalcified Bone Embedded in a Water-Miscible Plastic

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Authors

RFRichard M. FranklinRockefeller Foundation MMMaria‐Teresa MartinUniversity of Basel

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Implication

Experimental study demonstrates high-resolution sectioning and enzyme preservation in plastic-embedded undecalcified bone, suggesting improved histological and immunofluorescent evaluation.

Key Points

To develop and evaluate a low-temperature, water-miscible plastic embedding method for high-resolution sectioning, histochemistry, and immunostaining of undecalcified bone.
Embedded undecalcified bone fixed in various fixatives at 4 °C using a formulation of 2-hydroxypropyl methacrylate, 2-butoxyethanol plasticizer, and triethyleneglycol dimethacrylate cross-linker.
Cut sections at 1 to 5 µm thickness and tested histochemical stains, enzyme assays, and prestaining immunofluorescent labeling for collagen I and fibronectin.
Produced thin bone sections exhibiting minimal tissue compression, negligible wrinkling, and high cellular preservation.
Achieved successful staining with Gill's H&E, von Kossa, alizarin red, and Feulgen, while retaining strong acid and alkaline phosphatase activity alongside specific immunofluorescent antibody labeling.

Cite This Study

Franklin et al. (1980) studied this question.

synapsesocial.com/papers/6a7dbc8010b6f5f3737d8d7d https://doi.org/10.3109/10520298009067260

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Implication

Experimental study demonstrates high-resolution sectioning and enzyme preservation in plastic-embedded undecalcified bone, suggesting improved histological and immunofluorescent evaluation.

Staining and Histochemistry of Undecalcified Bone Embedded in a Water-Miscible Plastic

Discussion

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Implication

Key Points

Cite This Study

Staining and Histochemistry of Undecalcified Bone Embedded in a Water-Miscible Plastic

Discussion

Member takes

Implication

Key Points

Cite This Study

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