What question did this study set out to answer?

To describe standardized, reproducible protocols for the purification, culture, and CD19-CAR lentiviral engineering of natural killer cells isolated from adult peripheral blood and umbilical cord blood.

October 5, 2020Current Protocols in Immunology

Purification, Culture, and CD19‐CAR Lentiviral Transduction of Adult and Umbilical Cord Blood NK Cells

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Authors

LHLara HerreraBioCruces Health research Institute MJManel JuanHospital Sant Joan de Déu Barcelona CECristina EguizábalBioCruces Health research Institute

Discussion

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Implication

Laboratory protocol demonstrates efficient CD19-CAR lentiviral transduction of adult and cord blood natural killer cells, indicating an accessible pathway for off-the-shelf antitumor immunotherapies.

Key Points

To describe standardized, reproducible protocols for the purification, culture, and CD19-CAR lentiviral engineering of natural killer cells isolated from adult peripheral blood and umbilical cord blood.
Purification and culture protocols designed for primary natural killer cells derived from adult peripheral blood and umbilical cord blood.
Generation of lentiviral vector supernatants and subsequent transduction of natural killer cells to express CD19-targeted chimeric antigen receptors.
Established workflows enabling reliable ex vivo expansion and high-purity isolation of primary natural killer cells suitable for allogeneic therapeutic applications.
Optimized transduction parameters to achieve effective CD19-CAR gene transfer without inducing graft-versus-host alloreactivity.

Cite This Study

Herrera et al. (2020) studied this question.

synapsesocial.com/papers/6a7dd587629c956ef7ac86ea https://doi.org/10.1002/cpim.108

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Implication

Laboratory protocol demonstrates efficient CD19-CAR lentiviral transduction of adult and cord blood natural killer cells, indicating an accessible pathway for off-the-shelf antitumor immunotherapies.

Purification, Culture, and CD19‐CAR Lentiviral Transduction of Adult and Umbilical Cord Blood NK Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Purification, Culture, and CD19‐CAR Lentiviral Transduction of Adult and Umbilical Cord Blood NK Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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