Why the study?
Does an ELISA using recombinant VP2332-452 protein improve the detection of antibodies against Aleutian mink disease virus compared to CIEP and WB?
Does an ELISA using recombinant VP2332-452 protein improve the detection of antibodies against Aleutian mink disease virus compared to CIEP and WB?
The developed VP2332-452 ELISA offers high sensitivity and specificity for detecting AMDV antibodies, outperforming Western blotting.
For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.
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Chen et al. (2015) studied this question.
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