March 5, 2003The FASEB Journal

Besides affecting intracellular calcium signaling, 2‐APB reversibly blocks gap junctional coupling in confluent monolayers, thereby allowing the measurement of single‐cell membrane currents in undissociated cells

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Population

Monolayers of normal rat kidney cells (NRK/49F) and human embryonic kidney epithelial cells (HEK293/tsA201)

Comparison

2-aminoethoxydiphenyl borate applied… vs Absence of 2-APB or dissociated cells

Design

Preclinical

Authors

EHErik HarksSt Thomas' Hospital JCJesús P. CamiñaUniversidade de Santiago de Compostela PPPeter H. J. PetersRadboud University Nijmegen

Discussion

Member takes

Implication

Offers reversible uncoupler for in vitro gap-junction studies without cell dissociation; leaves open translation to native cardiac tissue or clinical use.

Structured PICO

Population

Monolayers of normal rat kidney cells (NRK/49F) and human embryonic kidney epithelial cells (HEK293/tsA201)

Intervention

2-aminoethoxydiphenyl borate (2-APB) applied extracellularly

Comparator

Absence of 2-APB or dissociated cells

Outcome

Gap junctional intercellular communication and electrical coupling

2-APB acts as an electrical uncoupler in confluent cell monolayers, providing a pharmacological tool to study single-cell membrane currents without prior cell dissociation.

Cite This Study

Harks et al. (2003) studied this question.

synapsesocial.com/papers/6a7df1eafd5c83ea4ea14775 https://doi.org/10.1096/fj.02-0786fje

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Implication

Offers reversible uncoupler for in vitro gap-junction studies without cell dissociation; leaves open translation to native cardiac tissue or clinical use.