September 1, 1999Journal of Biological ChemistryOpen Access

Thrombin Interacts with Thrombomodulin, Protein C, and Thrombin-activatable Fibrinolysis Inhibitor via Specific and Distinct Domains

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Population

56 purified thrombin mutants

Design

Preclinical

Authors

SHScott W. HallStanford University MNMariko NagashimaYamaguchi University LZLei ZhaoStanford University

Discussion

Member takes

Implication

May permit engineering of thrombin variants with dissociated activities; leaves open translation to human therapeutics.

Structured PICO

Population

56 purified thrombin mutants (in which 76 charged or polar surface residues on thrombin were mutated to alanine)

Intervention

Site-directed mutagenesis of thrombin surface residues

Outcome

Identification of key residues mediating the interactions of thrombin with thrombomodulin (TM), protein C, and thrombin-activatable fibrinolysis inhibitor (TAFI)surrogate

Distinct domains in thrombin mediate its interactions with thrombomodulin, protein C, and TAFI, allowing for the dissociation of its selective enzymatic properties via mutagenesis.

Cite This Study

Hall et al. (1999) studied this question.

synapsesocial.com/papers/6a7df86b7bc3bc414599b7e1 https://doi.org/10.1074/jbc.274.36.25510

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September 1, 1999Journal of Biological ChemistryOpen Access

Thrombin Interacts with Thrombomodulin, Protein C, and Thrombin-activatable Fibrinolysis Inhibitor via Specific and Distinct Domains

View Full Paper

Ask AI

Bookmark

Population

56 purified thrombin mutants

Design

Preclinical

Authors

SHScott W. HallStanford University MNMariko NagashimaYamaguchi University LZLei ZhaoStanford University

Discussion

Member takes

Implication

May permit engineering of thrombin variants with dissociated activities; leaves open translation to human therapeutics.

Structured PICO

Population

56 purified thrombin mutants (in which 76 charged or polar surface residues on thrombin were mutated to alanine)

Intervention

Site-directed mutagenesis of thrombin surface residues

Outcome

Identification of key residues mediating the interactions of thrombin with thrombomodulin (TM), protein C, and thrombin-activatable fibrinolysis inhibitor (TAFI)surrogate

Distinct domains in thrombin mediate its interactions with thrombomodulin, protein C, and TAFI, allowing for the dissociation of its selective enzymatic properties via mutagenesis.

Cite This Study

Hall et al. (1999) studied this question.

synapsesocial.com/papers/6a7df86b7bc3bc414599b7e1 https://doi.org/10.1074/jbc.274.36.25510

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

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