Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
April 1, 1983Journal of Biological ChemistryOpen Access

Cholinergic antagonism of beta-adrenergic stimulation of cardiac membrane protein phosphorylation in situ.

View Full Paper
Ask AI
Bookmark
Share

Authors

YIYasushi IwasaShinshu UniversityMHM. Marlene HoseyFresenius Medical Care (United States)

Discussion

Loading...

Member takes

Implication

Ex vivo experimental study demonstrates muscarinic cholinergic antagonism of beta-adrenergic membrane phosphorylation in cardiac tissue, indicating a mechanism for modulating cardiac contractile...

Key Points

  • Investigate the modulation of beta-adrenergic-stimulated cardiac membrane protein phosphorylation by muscarinic cholinergic agonists in intact heart tissue.
  • Treated 32P-loaded newborn chick heart preparations with the beta-adrenergic agonist isoproterenol, the muscarinic agonist oxotremorine, or both.
  • Isolated membrane fractions containing sarcolemma and sarcoplasmic reticulum to evaluate protein phosphorylation and molecular weight shifts via gel electrophoresis.
  • Isoproterenol stimulated the phosphorylation of a low molecular weight protein (presumed phospholamban/calciductin, 14–26 kDa) within 10 seconds, increasing its apparent molecular weight by approximately 1,000 Da.
  • Subsequent treatment with oxotremorine abolished isoproterenol-induced phosphorylation within 30 seconds (maximal at 2–5 minutes) and reversed the molecular weight increase.

Cite This Study

Iwasa et al. (1983) studied this question.

synapsesocial.com/papers/6a7e374cfff18652e232f319https://doi.org/10.1016/s0021-9258(18)32661-9
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cyclic adenosine monophosphate dependent and independent phosphorylation of sarcolemma membrane proteins in perfused rat heart1979 · 68 citations
  2. 2Stimulation of calcium accumulation in cardiac sarcolemma by protein kinase1976 · 86 citations
  3. 3Characterization of the intrinsic cAMP-dependent protein kinase activity and endogenous substrates in highly purified cardiac sarcolemmal vesicles.1982 · 84 citations
  4. 4Adenosine 3', 5'-Monophosphate-Dependent Membrane Phosphorylation1974 · 155 citations
  5. 5The Ca2+-pumping ATPase of heart sarcolemma. Characterization, calmodulin dependence, and partial purification.1981 · 475 citations