August 15, 2015International Journal of VirologyOpen Access

Rapid Molecular Detection of Genetically Diverted Foot and Mouth Disease Virus Serotype O During the Outbreak of 2012 in Egypt

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Population

Samples collected from gum, tongue epithelium, vesicular fluid, buccal and gum swabs, as well as saliva of…

Comparison

High-speed Reverse-Transcription Quantitative… vs Routine RT-qPCR

Design

Other

Authors

SRSahar Abd El RahmanMansoura University BHBernd HoffmannFriedrich-Loeffler-Institut BHBernd HaasFriedrich-Loeffler-Institut

Discussion

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Implication

High-speed RT-qPCR may speed FMDV outbreak confirmation; leaves open prospective validation for routine surveillance.

Structured PICO

Population

Samples collected from gum, tongue epithelium, vesicular fluid, buccal and gum swabs, as well as saliva of infected cattle, buffalo and sheep

Intervention

High-speed Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) using 'IRES1' and '3D-OIE' primers

Comparator

Routine RT-qPCR

Outcome

Identification of Foot and Mouth Disease Virus (FMDV)

High-speed RT-qPCR provides a rapid and reliable method for detecting FMDV, facilitating swift confirmation of outbreaks and characterization of new isolates.

Cite This Study

Rahman et al. (2015) studied this question.

synapsesocial.com/papers/6a7e5c507ddcd43fa24f9b73 https://doi.org/10.3923/ijv.2015.96.111

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Rapid Molecular Detection of Genetically Diverted Foot and Mouth Disease Virus Serotype O During the Outbreak of 2012 in Egypt

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Rapid Molecular Detection of Genetically Diverted Foot and Mouth Disease Virus Serotype O During the Outbreak of 2012 in Egypt

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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