Key result
Real-time RT-PCR provided a more sensitive and rapid detection of FMDV compared to the complement fixation test, identifying the virus in 13 of 20 in-contact cattle and 19 of 30 apparently healthy carrier cattle.
Population
60 samples from cattle in Egypt
Comparison
Real-time reverse transcriptase polymerase chain… vs Complement fixation test (CFT) and virus isolation
Design
Other
Authors
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rRT-PCR enables sensitive FMDV detection across infection stages in cattle; leaves open its role in routine surveillance pending validation.
Observational (n=60)
No
Real-time RT-PCR provides a more sensitive and rapid method for detecting FMDV in cattle compared to CFT.
EL-Shehawy et al. (2012) conducted an observational in Foot and Mouth Disease (FMD) (n=60). Real-time RT-PCR vs. Complement Fixation Test (CFT) and Virus Isolation was evaluated on Detection of FMDV in in-contact and apparently healthy carrier cattle. Real-time RT-PCR provided a more sensitive and rapid detection of FMDV compared to the complement fixation test, identifying the virus in 13 of 20 in-contact cattle and 19 of 30 apparently healthy carrier cattle.
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