What question did this study set out to answer?

The research aims to develop a method for analyzing chromatin states at genomic loci within individual cells.

Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy

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Randomized trial evaluates chromatin state variations in individual cells, indicating new insights into gene regulation.

The research aims to develop a method for analyzing chromatin states at genomic loci within individual cells.
Developed the SCEPTRE technique using Expansion Microscopy for multiplexed detection.
Visualized multiple histone modifications at non-repetitive genomic regions in single cells.
Examined the relationship between histone modifications and RNA polymerase II loading.
SCEPTRE successfully distinguished multiple histone modifications at a single housekeeping gene.
Quantification revealed differences in histone modification levels at developmentally-regulated genes.
Found extensive variability in epigenetic states between individual gene loci.

Woodworth et al. (2021) studied this question.

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