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August 14, 2026ACS ES&T Water

Framework forDeveloping a Simple Method of AnalyzingEnvironmental Bacterial Cells: Combining Single-Round DNA AptamerSelection with Gold Nanoparticle Sensor Development for Legionella pneumophila Detection

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Authors

KMKoji MatsunagaYTYuiko Tasaki‐HandaYSYota Suzuki

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Overview

Laboratory study demonstrates rapid Legionella pneumophila detection using single-round DNA aptamer selection and gold nanoparticles, highlighting a faster, low-cost environmental monitoring...

Key Points

  • Develop and validate a rapid, simplified framework combining single-round DNA aptamer selection with gold nanoparticle colorimetric sensing for detecting environmental pathogens like Legionella pneumophila.
  • Screened high-affinity DNA aptamer candidates against Legionella pneumophila using single-round polymer-enhanced capillary transient isotachophoresis (SR-PectI).
  • Engineered a colorimetric aptasensor by conjugating the selected candidate aptamers to gold nanoparticles (AuNPs).
  • Identified seven high-affinity aptamers with dissociation constants between 1.0 and 14 nmol L⁻¹, representing a 9-fold higher affinity than previously reported aptamers.
  • Achieved a detection limit of 1.1 × 10² cell equivalents of Legionella pneumophila within a 40-minute assay duration.

Cite This Study

Matsunaga et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec746b70b84ec8b9136bchttps://doi.org/10.1021/acsestwater.6c00237
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