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August 14, 2026Isotopes in Environmental and Health Studies

A multi-tracer approach using stable isotopes and elements to assess provenance of Arapaima gigas

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Authors

GFGilmar Antonio Silva FilhoFCFábio José Viana CostaCNClésia C. Nascentes

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Overview

Chemical profiling reveals accurate discrimination of wild and farmed Arapaima gigas, highlighting a robust framework for seafood traceability and fisheries conservation.

Key Points

  • To evaluate whether combining stable isotope ratios and trace element profiling can accurately determine the geographical provenance and production system (wild versus farmed) of Amazonian pirarucu (Arapaima gigas).
  • Analyzed dorsal muscle tissue from 79 reference fish of known origin, including farmed fish from intensive aquaculture and wild-caught fish from the Amazon and Tocantins–Araguaia river basins in Brazil.
  • Assessed 52 blind commercial samples purchased from retail establishments in the Brazilian Federal District to evaluate practical market utility.
  • Quantified δ¹³C, δ¹⁵N, and trace element mass fractions (including Cs and Sr) and modeled origin discrimination using linear discriminant analysis (LDA).
  • Stable isotope ratios, particularly δ¹³C, effectively separated farmed fish from wild populations across different river basins.
  • Trace element analysis showed significant differences in cesium (Cs) and strontium (Sr) concentrations between wild river basins, enabling complete discrimination of reference groups under cross-validation.
  • Commercial market samples exhibited widespread dispersion within the discriminant space, indicating heterogeneous sourcing and potential mixing across supply chains.

Cite This Study

Filho et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec775b70b84ec8b913cf2https://doi.org/10.1080/10256016.2026.2710121
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