Inhibition of Serum and Urine Amylase Activity in Pancreatitis with Hyperlipemia
Authors
Discussion
Loading...
Member takes
Implication
Clinical study reveals reversible amylase inhibition in serum and urine of patients with pancreatitis and hyperlipemia, indicating a circulating inhibitor distinct from lipids.
Key Points
- To examine the mechanisms of suppressed amylase activity and determine whether circulating inhibitors cause falsely normal readings in acute pancreatitis with hyperlipemia.
- Evaluated serum and urine amylase activity using serial dilution and ultracentrifugation across 7 patients with acute pancreatitis and hyperlipemia.
- Assayed control samples, including lipemic serum from patients without pancreatitis and pancreatitis serum enriched with exogenous lipids.
- Amylase activity inhibition occurred in 6 of 7 patients and resolved alongside declining triglyceride levels in 4 patients, though 2 showed no relationship with triglyceride levels.
- Ultracentrifugal lipid removal did not reverse amylase inhibition, and adding exogenous lipids to non-inhibited pancreatitis serum failed to replicate the inhibitory effect.
- Suppression of amylase activity also occurred in urine, indicating an independent circulating inhibitor rather than direct interference by serum lipids.
Cite This Study
Warshaw et al. (1975) studied this question.