Cln3 can work independently of Whi5 on the cell size for Start in yeast

Figure 1.. From top to bottom, Fig. 1A, 1B, and 1C, showing AS tetrads 9, 10, and 12, respectively. Identities, genotypes, and mean, median, and mode cell sizes of each spore clone are shown in Table S1. Cells were grown to early log phase (~ 2 × 10^7 cells/ml) and cell sizes in fL were measured using a Beckman-Coulter Multisizer 4e with a 50 micron aperture.

Figure 2.. Mean Cell Sizes Fig. 2A. Cells were grown to early log phase (~2 × 10^7 cells/ml) in YEPD. Cell sizes were measured with a Beckman-Coulter Multisizer with a 50 micron aperture, and mean cell sizes are shown. Each bar represents the mean of six technical replicates of a single strain of the stated genotype. Thus, the three wild-type bars represent a total of 18 measurements of three different but isogenic wild-type strains. The strains come from the AS9, AS10, and AS12 tetrads. The diploid was in the GZ background, and was a cln3/+ whi5/+ double heterozygote. Standard errors are shown numerically in Table S1. Fig. 2B. As Fig. 2A, but with strains from the BY background, measured with a Beckman-Coulter Z2 Coulter Counter with a 50 micron aperture. In this case, only 1 technical replicate was done. Numerical data is shown in Table S2. In 2A and 2B, the two diploid bars shown are not independent measurements, but are the same data added to both graphs for comparison.

Figure 3.. Cells in the BY background were grown to early log phase (~2 × 10^7 cells/ml) in YEPD, and spread on agar pads on microscope slides and photographed using Nomarski optics.

Figure 4.. WT and whi5 cells were grown to early log phase (~2 × 10^7 cells/ml) in YEPD and sizes were measured using a Beckman-Coulter Multisizer. The portion of the size distribution with cell sizes between 90 fL and 210 fL is shown.