What question did this study set out to answer?

To investigate the structural mechanisms and conformational rearrangements mediating pH-dependent gating in the KcsA potassium channel under near-physiological conditions.

March 14, 2007Journal of Biological ChemistryOpen Access

Identification and Characterization of the Slowly Exchanging pH-dependent Conformational Rearrangement in KcsA

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Authors

KTKoh TakeuchiKyoto University HTHideo TakahashiKindai University SKSeiko KawanoKurume University

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Implication

Solution-state NMR spectroscopy reveals pH-dependent conformational gating in KcsA channels, indicating dual gating governed by intracellular and filter components.

Key Points

To investigate the structural mechanisms and conformational rearrangements mediating pH-dependent gating in the KcsA potassium channel under near-physiological conditions.
Acquired series of 1H-15N-TROSY HSQC solution-state NMR spectra across varying pH levels from 3.9 to 5.2.
Evaluated resonance chemical shift perturbations in wild-type KcsA and a His-25 to Ala (H25A) substitution mutant.
Pronounced chemical shift changes occurred between pH 3.9 and 5.2 near the intracellular helix bundle and inner helix extension, while the H25A substitution completely abolished these structural rearrangements.
Conformational shifts in the Trp-67 indole alongside slow bundle kinetics support a dual-gating mechanism where activation-coupled inactivation at the selectivity filter regulates channel opening.

Cite This Study

Takeuchi et al. (2007) studied this question.

synapsesocial.com/papers/6a7f4ed6c4b89acbc5194479 https://doi.org/10.1074/jbc.m608264200

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Implication

Solution-state NMR spectroscopy reveals pH-dependent conformational gating in KcsA channels, indicating dual gating governed by intracellular and filter components.

Identification and Characterization of the Slowly Exchanging pH-dependent Conformational Rearrangement in KcsA

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification and Characterization of the Slowly Exchanging pH-dependent Conformational Rearrangement in KcsA

Discussion

Member takes

Implication

Key Points

Cite This Study

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