April 15, 2004BiochemistryOpen Access

Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Kinetic, Thermodynamic, and Structural Analysis of Ribonucleotide Selection

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Population

Poliovirus RNA-Dependent RNA Polymerase (3Dpol) derivatives

Design

Preclinical

Authors

DGDavid W. GoharaClinical Research Consultants (United States)JAJamie J. ArnoldUniversity of North Carolina at Chapel Hill CCCraig E. CameronUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Asp-238 mechanism may guide RdRp-targeted antivirals; leaves open translation beyond structural models.

Structured PICO

Population

Poliovirus RNA-Dependent RNA Polymerase (3Dpol) derivatives

Intervention

Substitutions in the ribose-binding pocket with ATP analogues containing correct and incorrect sugar configurations

Outcome

Kinetic and thermodynamic analysis of ribonucleotide selection and polymerase fidelity

Asp-238 in structural motif A is a key determinant of polymerase fidelity in poliovirus RNA-dependent RNA polymerase, a mechanism likely conserved across all RNA-dependent RNA polymerases.

Cite This Study

Gohara et al. (2004) studied this question.

synapsesocial.com/papers/6a7f4f64aa1ae1567cb3d794 https://doi.org/10.1021/bi035429s

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Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Kinetic, Thermodynamic, and Structural Analysis of Ribonucleotide Selection

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Kinetic, Thermodynamic, and Structural Analysis of Ribonucleotide Selection

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider