Key result
The Glu-297 mutation in poliovirus RNA-dependent RNA polymerase reduced ribonucleotide misincorporation fidelity by 14-fold relative to wild type and prevented viral growth in HeLa cells.
The study identifies the first RdRp derivative with a mutator phenotype, providing a mechanistic basis for elevated mutation frequencies in RNA phages compared to animal RNA viruses.
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Identifies first mutator RdRp phenotype; extends mechanistic understanding of viral mutation rates but leaves open in vivo validation.
Korneeva et al. (2007) studied Poliovirus replication. Glu-297 mutation in 3Dpol vs. Wild-type 3Dpol was evaluated on Polymerase fidelity and viral replication. The Glu-297 mutation in poliovirus RNA-dependent RNA polymerase reduced ribonucleotide misincorporation fidelity by 14-fold relative to wild type and prevented viral growth in HeLa cells.
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