January 7, 1997Proceedings of the National Academy of SciencesOpen Access

Spare the rod, spoil the regulation: Necessity for a myosin rod

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Population

Expressed vertebrate smooth muscle myosin subfragments and mutants

Comparison

Mutants with varying lengths of rod sequence and… vs Myosin subfragments with up to 100 amino acids…

Design

Preclinical

Authors

KTKathleen M. TrybusUniversity of Vermont YFYelena FreyzonBroad Institute LFLynn Z. FaustSouth University

Discussion

Member takes

Implication

Rod length and dimerization enable full myosin inactivation in vitro; extends molecular insights but leaves clinical relevance open.

Structured PICO

Population

Expressed vertebrate smooth muscle myosin subfragments and mutants

Intervention

Mutants with varying lengths of rod sequence and C-terminal leucine zippers, or addition of native rod sequence

Comparator

Myosin subfragments with up to 100 amino acids of coiled-coil rod sequence without dimerization

Outcome

Regulation of ATPase activity (ability to achieve an 'off' state)surrogate

The myosin rod mediates specific interactions with the head that are required to obtain the completely inactive state of vertebrate smooth and non-muscle myosins.

Cite This Study

Trybus et al. (1997) studied this question.

synapsesocial.com/papers/6a7f9aa5fcb7d43d28406a6a https://doi.org/10.1073/pnas.94.1.48

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Spare the rod, spoil the regulation: Necessity for a myosin rod

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Spare the rod, spoil the regulation: Necessity for a myosin rod

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider