October 1, 1996Journal of VirologyOpen Access

Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers

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Population

brome mosaic virus RNA-dependent RNA polymerase (RdRp)

Comparison

guanosine monophosphate or specific… vs reactions performed with limited GTP…

Design

Preclinical

Authors

CKChia‐Sui KaoCleveland Clinic JSJin-Hua SunUniversity of Science and Technology of China

Discussion

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Implication

GpG may prime BMV RdRp to boost minus-strand synthesis; leaves open extension to other viruses or antiviral targeting.

Structured PICO

Population

brome mosaic virus RNA-dependent RNA polymerase (RdRp)

Intervention

guanosine monophosphate or specific oligoribonucleotides (e.g., guanylyl-3',5'-guanosine (GpG))

Comparator

reactions performed with limited GTP concentrations without primers

Outcome

minus-strand RNA synthesis rate and Km for GTPsurrogate

Guanylyl-3',5'-guanosine (GpG) acts as a primer for brome mosaic virus RdRp, significantly decreasing its Km for GTP and increasing the rate of minus-strand RNA synthesis.

Cite This Study

Kao et al. (1996) studied this question.

synapsesocial.com/papers/6a7fb2a137804d00d37852aa https://doi.org/10.1128/jvi.70.10.6826-6830.1996

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Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider