Draft Genome Sequence Analysis of the Genotype II African Swine Fever Virus from India

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  FIG 1. Phylogenetic analysis of ASFV based on B646L and E183L genes. Phylogenetic analysis was done in MEGA X software, and multiple sequence alignment of the nucleotide sequences was done with the CLUSTALW program. The phylogenetic trees were constructed by the neighbor-joining (NJ) method using a Kimura 2-parameter substitution model, and statistical significance was determined by 1,000 bootstraps. Gene sequences of different ASFV strains are represented by GenBank accession numbers with respective genotypes in parentheses. (a) A phylogenetic tree generated with a partial B646L gene and the ASFV strain ABTCVSCK_ASF007 (accession number OM481276.1, marked with a solid red circle) formed a clade with genotype II. (b) A phylogenetic tree constructed with the E183L gene also confirmed that the ASFV strain considered in this study belongs to genotype II of ASFV (highlighted with a solid red circle).