What question did this study set out to answer?

To examine the binding affinity and specificity of lipocalin-type prostaglandin D synthase toward bile pigments, thyroid hormones, steroid hormones, and fatty acids.

June 1, 1999Biochemistry

Binding of Biliverdin, Bilirubin, and Thyroid Hormones to Lipocalin-Type Prostaglandin D Synthase

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Authors

CBCarsten T. BeuckmannEisai (Japan)MAMasaaki Aoyagi IOIssay OkazakiOsaka Science Museum

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Implication

In vitro analysis demonstrates binding of bile pigments and thyroid hormones to lipocalin-type prostaglandin D synthase, indicating distinct ligand transport specificity among lipocalins.

Key Points

To examine the binding affinity and specificity of lipocalin-type prostaglandin D synthase toward bile pigments, thyroid hormones, steroid hormones, and fatty acids.
Assayed recombinant lipocalin-type prostaglandin D synthase using tryptophan fluorescence quenching, circular dichroism, resonant mirror technique, and surface plasmon resonance.
Tested enzymatic inhibition kinetics and evaluated ligand-binding interactions with control lipocalins, including epididymal retinoic acid-binding protein and beta-lactoglobulin.
Prostaglandin D synthase bound ligands with dissociation constants of 33 nM for biliverdin, 37 nM for bilirubin, 660 nM for L-thyroxine, 820 nM for 3,3',5'-triiodo-L-thyronine, and 2.08 µM for 3,3',5-triiodo-L-thyronine.
Ligand binding shifted absorption peaks from 375 to 380 nm for biliverdin and 439 to 446 nm for bilirubin, while noncompetitively inhibiting enzyme activity with IC50 values between 3.9 and 10.9 µM.
Epididymal retinoic acid-binding protein and beta-lactoglobulin exhibited no significant binding to bile pigments or thyroid hormones.

Cite This Study

Beuckmann et al. (1999) studied this question.

synapsesocial.com/papers/6a80d3ea1ece0ba82ce18bae https://doi.org/10.1021/bi990261p

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Implication

In vitro analysis demonstrates binding of bile pigments and thyroid hormones to lipocalin-type prostaglandin D synthase, indicating distinct ligand transport specificity among lipocalins.

Binding of Biliverdin, Bilirubin, and Thyroid Hormones to Lipocalin-Type Prostaglandin D Synthase

Discussion

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Implication

Key Points

Cite This Study

Binding of Biliverdin, Bilirubin, and Thyroid Hormones to Lipocalin-Type Prostaglandin D Synthase

Discussion

Member takes

Implication

Key Points

Cite This Study

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