What question did this study set out to answer?

To characterize the pharmacological properties and identify selective inhibitors of swelling-induced chloride currents in canine atrial myocytes.

December 1, 1994Journal of Cardiovascular Electrophysiology

Pharmacologic Properties of the Swelling‐Induced Chloride Current of Dog Atrial Myocytes

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Population

Dog atrial cells (myocytes)

Design

Preclinical

Authors

SSSteve SorotaNew England Biolabs (United States)

Discussion

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Implication

May aid mechanistic studies of atrial Cl− currents in models; leaves open clinical translation given animal data and nonspecific effects.

Key Points

To characterize the pharmacological properties and identify selective inhibitors of swelling-induced chloride currents in canine atrial myocytes.
Utilized whole-cell patch clamp electrophysiology to measure membrane currents in isolated dog atrial myocytes.
Activated chloride currents using osmotic swelling stress and tested current selectivity, calcium dependence, and sensitivity to various chloride channel blockers.
Niflumic acid (100 µM), NPPB (10–40 µM), and IAA-94 (100 µM) completely blocked the swelling-induced chloride current without decreasing cell volume, though nonspecific effects were detected.
Anthracene-9-carboxylic acid (1 mM), dideoxyforskolin (100 µM), and extracellular cAMP (5 mM) produced partial inhibition of approximately 50%, 80%, and 10%, respectively, whereas DIDS (100 µM) and DNDS (5 mM) selectively abolished outward currents.

Structured PICO

Population

Dog atrial cells (myocytes)

Intervention

Putative chloride channel blockers including Anthracene-9-carboxylic acid, dideoxyforskolin, extracellular cAMP, Niflumic acid, NPPB, IAA-94, DIDS, and DNDS

Outcome

Inhibition of swelling-induced chloride current measured via whole cell patch clampsurrogate

Niflumic acid, IAA-94, and NPPB are full blockers of cardiac swelling-induced chloride currents in dog atrial cells, but their nonspecific effects necessitate caution in experimental use.

Limitations

Nonspecific effects were identified for each of the full blockers

Cite This Study

Steve Sorota (1994) studied this question.

synapsesocial.com/papers/6a80d900fd865eccc665dcbb https://doi.org/10.1111/j.1540-8167.1994.tb01143.x

View Full Paper

Ask AI

Bookmark

Implication

May aid mechanistic studies of atrial Cl− currents in models; leaves open clinical translation given animal data and nonspecific effects.

Pharmacologic Properties of the Swelling‐Induced Chloride Current of Dog Atrial Myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Pharmacologic Properties of the Swelling‐Induced Chloride Current of Dog Atrial Myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider